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KMID : 0613820200300050420
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Journal of Life Science
2020 Volume.30 No. 5 p.420 ~ p.427
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Improvement of Artificial Antibody Secretion Using Supercharged Protein
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Park Ji-Yeon
Choi Hee-Ju
Lee Hye-Jin
Ahn Jung-Hoon
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Abstract
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A repebody, an artificial non-immunoglobulin protein scaffold, is expected to be a solution in the search for faster, cheaper, and customizable antibodies. However, the production of medical repebodies remains difficult due to their low yield and the complex purification processes required. The Pseudomonas fluorescens ABC transporter system has been suggested as an efficient and cost-effective method for repebody production, but the total yield is low because of the secreted protein’s positive charge; thus, a repebody with a high isoelectric point needs to be changed into a more negatively charged protein for better secretion. To achieve this, we first attached oligo-aspartic acids to the N- and C-terminals of the repebody, but secretion efficiency was not enhanced significantly. Subsequently, we devised an alternative method for improved secretion efficiency by engineering fifteen positively charged amino acids to aspartic acid in the non-antigen binding sites of the repebody to give a high net negative charge. As a result, secretion efficiency was greatly enhanced from 21.2% (wildtype) to 58.5% (negatively supercharged). The negatively supercharged repebody was succussfully produced extracellularly by ABC transporter secretion system in P. fluorescens.
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KEYWORD
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ABC transporter, Isoelectric point (pI), negatively supercharged, Pseudomonas fluorescens, repebody
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